Archaeological Investigations within San Pedro Springs Park (41BX19), San Antonio, Bexar County, Texas

The University of Texas at San Antonio Center for Archaeological Research (UTSA-CAR) contracted with Adams Environmental, Inc. to provide archaeological services to Capital Improvement Management (CIMS) of the City of San Antonio (COSA) related to the archaeological investigation of selected areas of San Pedro Springs Park in San Antonio, Bexar County, Texas. The CAR conducted archaeological testing at this National Register Site, 41BX19, from early December 2013 to mid-January of 2014. The goals of archaeological investigations were to identify and investigate any proto-historic and historic archaeological deposits associated with Colonial Period occupants of the area, including evidence of the first acequia and associated dam, and the location of the first presidio and villa. In addition, CAR was tasked with the investigation of any prehistoric cultural deposits encountered. This project was performed by staff archaeologists from the CAR. It was conducted under Texas Antiquities Permit No. 6727, with Dr. Steve Tomka serving as Principal Investigator (PI), and Kristi Nichols and Stephen Smith serving as Project Archaeologists. Dr. Tomka departed from UTSA shortly after the completion of fieldwork. At that time, Dr. Raymond Mauldin of CAR assumed PI responsibilities for the project. One hundred and eleven shovel tests, eleven 1-x-1 m test units, two 50-x-50 cm units, two backhoe trenches, and several auger holes were excavated during this effort. Minimal artifactual evidence of colonial occupants was noted during the archaeological investigations. Several Native American bone tempered sherds that could reflect either Late Prehistoric Leon Plain or Goliad ware were recovered. However, no Spanish Majolicas or lead glazed wares were uncovered, and no gunflints were identified in the lithic assemblage. Due to various utility lines and other obstructions, backhoe trenches to search for the acequia and associated dam could not be excavated. It is likely that areas proposed for investigation of the acequia and associated dam have been disturbed by aforementioned utility lines as well as earlier construction within the park. No evidence of the specific location of the first presidio or villa was located. Shovel testing and test units revealed the presence of historic and prehistoric use of the park, though mixing of historic and prehistoric material, as well as other disturbances (e.g., rodents), was common in the deposits. However, there was an increase in prehistoric material with depth as revealed in shovel testing results. Shovel testing located Feature 1, a burned rock feature that possibly was associated with a sheet midden, as well as several areas with high densities of prehistoric materials. Test excavations, based on these shovel tests, suggest that Feature 1 is a discrete feature that lies below a widespread, low-density distribution of burned rock. Shovel testing also identified a high-density cluster of lithic, bone, and burned rock. The excavation of a 1-x-1 m test unit (TU 4) in this area produced over 4,000 pieces of debitage, with over 50% of this total coming from three levels. Burned rock, a variety of tools, faunal material, and charcoal were present throughout these levels. Temporal placement of deposits relied on artifact typologies (e.g., ceramic types, lithic projectile points, lithic tool types) as well as two charcoal and four bone collagen radiocarbon dates. Artifact typologies suggest occupation as early as the Early Archaic as reflected by a possible Guadalupe tool. A series of Late Archaic Points (Castroville, Frio, Marcos, and Montell) and Late Prehistoric point forms (Edwards, Perdiz, and Scallorn) are present from several areas. In addition, a possible Middle Archaic La Jita point was recovered. The bone tempered Native American wares could date as early as AD 1250, though they could also reflect proto-historic or colonial age materials. Other ceramics primarily suggest a mid-nineteenth- to midtwentieth- century occupation. Using the midpoints of the 1-sigma distribution, calibrated radiocarbon dates show use of San Pedro Park from as early as 100 AD (CAR 345; 1905 +/- 22 Radiocarbon Years Before Present [RCYBP]) to as recently as the early twentieth century. The more recent end of that range is a function of two late dates from two different areas of the park. The first of these is on a bison bone (CAR 344) that returned a date of 158 +/- 23 RCYBP. The second is on a bone consistent with a bison-sized animal (CAR 346) that produced a date of 155 +/- 23 RCYBP. The corrected, calibrated dates for these two samples range from AD 1670 to the early 1940s using the 1-sigma spread. The wide range of these dates is related to the flat calibration curve late in time. However, the most probable date range (ca. 36% probability) for these two dates is between AD 1729 and 1779, with a roughly 48% probability that they date prior to AD 1779. Limited testing suggests that, with a few specific exceptions, the upper 30-40 cm of San Pedro Park is extensively disturbed. However, though some disturbances are present, at least three areas have materials in what appears to be good context. These include material dating to the Late Archaic, Late Prehistoric, and possibly the Proto-historic or Colonial Period. Based on historic maps, previous work, and the current investigation, CAR proposes a series of management areas for San Pedro Park. If work in these management areas follows these suggestions for various limits on subsurface impacts, CAR recommends that renovation activities within the park be allowed to proceed. The Texas Historical Commission (THC), in a letter dated February 4, 2015, agreed with these recommendations. Finally, CAR provides several recommendations for public education facilities within the park. In accordance with the THC Permit specifications and the Scope of Work for this project, all field notes, analytical notes, photographs, and other project related documents, along with a copy of the final report, will be curated at the CAR. After quantification and completion of analysis, and in consultation with THC and the COSA Office of Historic Preservation, artifacts possessing little scientific value were discarded pursuant to Chapter 26.27(g)(2) of the Antiquities Code of Texas. Artifact classes discarded specific to this project included samples of burned rock and snail shell, all unidentifiable metal, soil samples, and recent (post-1950) material

KRT5 missense variant in a Cardigan Welsh Corgi with epidermolysis bullosa simplex.

Epidermolysis bullosa (EB) is a group of blistering disorders that includes several subtypes, classified according to their level of cleavage. Typical clinical signs are blisters and erosions resulting from minimal trauma. The disease has been described in many mammalian species and pathogenic variants in at least 18 different genes have been identified. In the present study, we investigated a Cardigan Welsh Corgi with congenital clinical signs consistent with epidermolysis bullosa. The puppy had blisters and erosions on the paw pads, and the oral mucosa. Histologic examination demonstrated the typical clefting between the dermis and epidermis and confirmed the clinical suspicion. We obtained whole genome sequencing data from the affected puppy and searched for variants in candidate genes known to cause EB. This revealed a heterozygous missense variant, KRT5:p.(E476K), affecting the highly conserved KLLEGE motif of keratin 5. The mutant allele in the affected puppy arose owing to a de novo mutation event as it was absent from both unaffected parents. Knowledge of the functional impact of KRT5 variants in other species together with the demonstration of the de novo mutation event establishes KRT5:p.(E476K) as causative variant for the observed EBS

ABHD5 frameshift deletion in Golden Retrievers with ichthyosis.

Ichthyoses are hereditary skin disorders characterized by the formation of scales and defects in the outermost layer of the epidermis. In dogs, at least six different breed-specific ichthyoses including a relatively common PNPLA1-related autosomal recessive ichthyosis in Golden Retrievers are known. In this study, we investigated 14 Golden Retrievers with scales that were not homozygous for the mutant PNPLA1 allele suggesting a genetically distinct new form of ichthyosis. Histopathological examinations showed lamellar, orthokeratotic hyperkeratosis, and mildly hyperplastic epidermis that led to the diagnosis of a nonepidermolytic ichthyosis. Combined linkage and homozygosity mapping in 14 cases and 30 nonaffected family members delimited a critical interval of ∼12.7 Mb on chromosome 23. Whole-genome sequencing of an affected dog revealed a single protein-changing variant within this region that was not present in 795 control genomes. The identified variant is a 14 bp deletion in the ABHD5 gene (c.1006_1019del), leading to a frameshift and altering the last 14 codons p.(Asp336Serfs*6). The genotypes at this variant showed perfect cosegregation with the ichthyosis phenotype in a large family comprising 14 cases and 72 controls. ABHD5 encodes an acyltransferase required for lipid metabolism. In humans, variants in ABHD5 cause Chanarin-Dorfman syndrome, a neutral lipid storage disease with ichthyosis. Our data in dogs together with the knowledge on the effects of ABHD5 variants in humans strongly suggest ABHD5:c.1006_1019del as candidate causative genetic variant for a new canine form of ichthyosis, which we propose to designate as Golden Retriever ichthyosis type 2 (ICH2)

Independent COL17A1 Variants in Cats with Junctional Epidermolysis Bullosa.

Epidermolysis bullosa (EB), characterized by defective adhesion of the epidermis to the dermis, is a heterogeneous disease with many subtypes in human patients and domestic animals. We investigated two unrelated cats with recurring erosions and ulcers on ear pinnae, oral mucosa, and paw pads that were suggestive of EB. Histopathology confirmed the diagnosis of EB in both cats. Case 1 was severe and had to be euthanized at 5 months of age. Case 2 had a milder course and was alive at 11 years of age at the time of writing. Whole genome sequencing of both affected cats revealed independent homozygous variants in COL17A1 encoding the collagen type XVII alpha 1 chain. Loss of function variants in COL17A1 lead to junctional epidermolysis bullosa (JEB) in human patients. The identified splice site variant in case 1, c.3019+1del, was predicted to lead to a complete deficiency in collagen type XVII. Case 2 had a splice region variant, c.769+5G>A. Assessment of the functional impact of this variant on the transcript level demonstrated partial aberrant splicing with residual expression of wildtype transcript. Thus, the molecular analyses provided a plausible explanation of the difference in clinical severity between the two cases and allowed the refinement of the diagnosis in the affected cats to JEB. This study highlights the complexity of EB in animals and contributes to a better understanding of the genotype-phenotype correlation in COL17A1-related JEB

Archaeological Investigations within San Pedro Springs Park (41BX19), San Antonio, Bexar County, Texas

The University of Texas at San Antonio Center for Archaeological Research (UTSA-CAR) contracted with Adams Environmental, Inc. to provide archaeological services to Capital Improvement Management (CIMS) of the City of San Antonio (COSA) related to the archaeological investigation of selected areas of San Pedro Springs Park in San Antonio, Bexar County, Texas. The CAR conducted archaeological testing at this National Register Site, 41BX19, from early December 2013 to mid-January of 2014. The goals of archaeological investigations were to identify and investigate any proto-historic and historic archaeological deposits associated with Colonial Period occupants of the area, including evidence of the first acequia and associated dam, and the location of the first presidio and villa. In addition, CAR was tasked with the investigation of any prehistoric cultural deposits encountered. This project was performed by staff archaeologists from the CAR. It was conducted under Texas Antiquities Permit No. 6727, with Dr. Steve Tomka serving as Principal Investigator (PI), and Kristi Nichols and Stephen Smith serving as Project Archaeologists. Dr. Tomka departed from UTSA shortly after the completion of fieldwork. At that time, Dr. Raymond Mauldin of CAR assumed PI responsibilities for the project. One hundred and eleven shovel tests, eleven 1-x-1 m test units, two 50-x-50 cm units, two backhoe trenches, and several auger holes were excavated during this effort. Minimal artifactual evidence of colonial occupants was noted during the archaeological investigations. Several Native American bone tempered sherds that could reflect either Late Prehistoric Leon Plain or Goliad ware were recovered. However, no Spanish Majolicas or lead glazed wares were uncovered, and no gunflints were identified in the lithic assemblage. Due to various utility lines and other obstructions, backhoe trenches to search for the acequia and associated dam could not be excavated. It is likely that areas proposed for investigation of the acequia and associated dam have been disturbed by aforementioned utility lines as well as earlier construction within the park. No evidence of the specific location of the first presidio or villa was located. Shovel testing and test units revealed the presence of historic and prehistoric use of the park, though mixing of historic and prehistoric material, as well as other disturbances (e.g., rodents), was common in the deposits. However, there was an increase in prehistoric material with depth as revealed in shovel testing results. Shovel testing located Feature 1, a burned rock feature that possibly was associated with a sheet midden, as well as several areas with high densities of prehistoric materials. Test excavations, based on these shovel tests, suggest that Feature 1 is a discrete feature that lies below a widespread, low-density distribution of burned rock. Shovel testing also identified a high-density cluster of lithic, bone, and burned rock. The excavation of a 1-x-1 m test unit (TU 4) in this area produced over 4,000 pieces of debitage, with over 50% of this total coming from three levels. Burned rock, a variety of tools, faunal material, and charcoal were present throughout these levels. Temporal placement of deposits relied on artifact typologies (e.g., ceramic types, lithic projectile points, lithic tool types) as well as two charcoal and four bone collagen radiocarbon dates. Artifact typologies suggest occupation as early as the Early Archaic as reflected by a possible Guadalupe tool. A series of Late Archaic Points (Castroville, Frio, Marcos, and Montell) and Late Prehistoric point forms (Edwards, Perdiz, and Scallorn) are present from several areas. In addition, a possible Middle Archaic La Jita point was recovered. The bone tempered Native American wares could date as early as AD 1250, though they could also reflect proto-historic or colonial age materials. Other ceramics primarily suggest a mid-nineteenth- to midtwentieth-century occupation. Using the midpoints of the 1-sigma distribution, calibrated radiocarbon dates show use of San Pedro Park from as early as 100 AD (CAR 345; 1905 +/- 22 Radiocarbon Years Before Present [RCYBP]) to as recently as the early twentieth century. The more recent end of that range is a function of two late dates from two different areas of the park. The first of these is on a bison bone (CAR 344) that returned a date of 158 +/- 23 RCYBP. The second is on a bone consistent with a bison-sized animal (CAR 346) that produced a date of 155 +/- 23 RCYBP. The corrected, calibrated dates for these two samples range from AD 1670 to the early 1940s using the 1-sigma spread. The wide range of these dates is related to the flat calibration curve late in time. However, the most probable date range (ca. 36% probability) for these two dates is between AD 1729 and 1779, with a roughly 48% probability that they date prior to AD 1779. Limited testing suggests that, with a few specific exceptions, the upper 30-40 cm of San Pedro Park is extensively disturbed. However, though some disturbances are present, at least three areas have materials in what appears to be good context. These include material dating to the Late Archaic, Late Prehistoric, and possibly the Proto-historic or Colonial Period. Based on historic maps, previous work, and the current investigation, CAR proposes a series of management areas for San Pedro Park. If work in these management areas follows these suggestions for various limits on subsurface impacts, CAR recommends that. renovation activities within the park be allowed to proceed. The Texas Historical Commission (THC), in a letter dated February 4, 2015, agreed with these recommendations. Finally, CAR provides several recommendations for public education facilities within the park. In accordance with the THC Permit specifications and the Scope of Work for this project, all field notes, analytical notes, photographs, and other project related documents, along with a copy of the final report, will be curated at the CAR. After quantification and completion of analysis, and in consultation with THC and the COSA Office of Historic Preservation, artifacts possessing little scientific value were discarded pursuant to Chapter 26.27(g)(2) of the Antiquities Code of Texas. Artifact classes discarded specific to this project included samples of burned rock and snail shell, all unidentifiable metal, soil samples, and recent (post-1950) material

Addressing Systemic Factors Related to Racial and Ethnic Disparities among Older Adults in Long-Term Care Facilities

Disparities in older adults’ care and experiences in long-term care facilities (LTCFs) such as nursing homes and assisted living/residential care communities reflect disparities in the broader society. Various policies and institutional practices related to economic opportunity, education, housing, health care, and retirement financing have created and maintain inequitable social structures in the United States. This chapter describes racial and ethnic disparities among older adults in LTCFs in the United States and the systemic factors associated with those disparities. It presents a conceptual framework for understanding the role of structural racism in the racial and ethnic inequities experienced by LTCF residents. In the framework, structural racism directly contributes to racial and ethnic inequities among LTCF residents through LTCF-related policies and practices. Structural racism also indirectly causes disparities among LTCF residents through health and economic disparities. The chapter describes current efforts that address the effects of structural racism within LTCFs and concludes with practice and policy recommendations to redress racial and ethnic disparities among LTCF residents

Spacecraft Dormancy Autonomy Analysis for a Crewed Martian Mission

Current concepts of operations for human exploration of Mars center on the staged deployment of spacecraft, logistics, and crew. Though most studies focus on the needs for human occupation of the spacecraft and habitats, these resources will spend most of their lifetime unoccupied. As such, it is important to identify the operational state of the unoccupied spacecraft or habitat, as well as to design the systems to enable the appropriate level of autonomy. Key goals for this study include providing a realistic assessment of what "dormancy" entails for human spacecraft, exploring gaps in state-of-the-art for autonomy in human spacecraft design, providing recommendations for investments in autonomous systems technology development, and developing architectural requirements for spacecraft that must be autonomous during dormant operations. The mission that was chosen is based on a crewed mission to Mars. In particular, this study focuses on the time that the spacecraft that carried humans to Mars spends dormant in Martian orbit while the crew carries out a surface mission. Communications constraints are assumed to be severe, with limited bandwidth and limited ability to send commands and receive telemetry. The assumptions made as part of this mission have close parallels with mission scenarios envisioned for dormant cis-lunar habitats that are stepping-stones to Mars missions. As such, the data in this report is expected to be broadly applicable to all dormant deep space human spacecraft

A Missense Variant Affecting the C-Terminal Tail of UNC93B1 in Dogs with Exfoliative Cutaneous Lupus Erythematosus (ECLE)

Cutaneous lupus erythematosus (CLE) in humans encompasses multiple subtypes that exhibit a wide array of skin lesions and, in some cases, are associated with the development of systemic lupus erythematosus (SLE). We investigated dogs with exfoliative cutaneous lupus erythematosus (ECLE), a dog-specific form of chronic CLE that is inherited as a monogenic autosomal recessive trait. A genome-wide association study (GWAS) with 14 cases and 29 controls confirmed a previously published result that the causative variant maps to chromosome 18. Autozygosity mapping refined the ECLE locus to a 493 kb critical interval. Filtering of whole genome sequence data from two cases against 654 controls revealed a single private protein-changing variant in this critical interval, UNC93B1:c.1438C>A or p.Pro480Thr. The homozygous mutant genotype was exclusively observed in 23 ECLE affected German Shorthaired Pointers and an ECLE affected Vizsla, but absent from 845 controls. UNC93B1 is a transmembrane protein located in the endoplasmic reticulum and endolysosomes, which is required for correct trafficking of several Toll-like receptors (TLRs). The p.Pro480Thr variant is predicted to affect the C-terminal tail of the UNC93B1 that has recently been shown to restrict TLR7 mediated autoimmunity via an interaction with syndecan binding protein (SDCBP). The functional knowledge on UNC93B1 strongly suggests that p.Pro480Thr is causing ECLE in dogs. These dogs therefore represent an interesting spontaneous model for human lupus erythematosus. Our results warrant further investigations of whether genetic variants affecting the C-terminus of UNC93B1 might be involved in specific subsets of CLE or SLE cases in humans and other species

A Missense Variant Affecting the C-Terminal Tail of UNC93B1 in Dogs with Exfoliative Cutaneous Lupus Erythematosus (ECLE)

Cutaneous lupus erythematosus (CLE) in humans encompasses multiple subtypes that exhibit a wide array of skin lesions and, in some cases, are associated with the development of systemic lupus erythematosus (SLE). We investigated dogs with exfoliative cutaneous lupus erythematosus (ECLE), a dog-specific form of chronic CLE that is inherited as a monogenic autosomal recessive trait. A genome-wide association study (GWAS) with 14 cases and 29 controls confirmed a previously published result that the causative variant maps to chromosome 18. Autozygosity mapping refined the ECLE locus to a 493 kb critical interval. Filtering of whole genome sequence data from two cases against 654 controls revealed a single private protein-changing variant in this critical interval, UNC93B1:c.1438C>A or p.Pro480Thr. The homozygous mutant genotype was exclusively observed in 23 ECLE affected German Shorthaired Pointers and an ECLE affected Vizsla, but absent from 845 controls. UNC93B1 is a transmembrane protein located in the endoplasmic reticulum and endolysosomes, which is required for correct trafficking of several Toll-like receptors (TLRs). The p.Pro480Thr variant is predicted to affect the C-terminal tail of the UNC93B1 that has recently been shown to restrict TLR7 mediated autoimmunity via an interaction with syndecan binding protein (SDCBP). The functional knowledge on UNC93B1 strongly suggests that p.Pro480Thr is causing ECLE in dogs. These dogs therefore represent an interesting spontaneous model for human lupus erythematosus. Our results warrant further investigations of whether genetic variants affecting the C-terminus of UNC93B1 might be involved in specific subsets of CLE or SLE cases in humans and other species

Southeastern Association of Law Libraries Annual Meeting

The 2009 SEAALL Annual Meeting was held in Athens Georgia, April 16-18, 2009